Replication of the complex composition and three-dimensionality of native extracellular matrix (ECM) in in vitro setting is highly desirable to boost the functionality of cell models. One of the most straightforward, and at the same time most technically challenging approaches, is the isolation of ECM by decellularization of tissues or organs. Indeed, most methods of decellularization result in disruption of the architecture and loss of structure and composition of native ECM.
Our group was particularly successful in the efficient and fast decellularization of the liver (Fig. 1 and 2), while preserving ECM integrity, including 3D structure, intact vasculature as well as presence of structural proteins and ECM-bound growth factors. Indeed, effective organ decellularization is dictated by factors such as tissue density and organization, geometric and biologic properties of desired end product and its targeted application. We would be happy to share with you our experiences with organ decellularization by e.g. helping you to optimize the decellularization process of the organ of your choice, either on the fee-for-service or collaborative basis. We presently have such collaborative project in the area of testis decellularization.
Interested parties can contact: